Detection of Aspergillus Fumigatus from Coriolis air samples by QPCR - Bertin Instruments

    Biomedical & Health

    Detection of Aspergillus Fumigatus from Coriolis air samples by QPCR

    Sources: Univ. Hospital of Besançon, and CNRS, Besançon, France

    Context

    Fungal infections represent a constant threat in hospitals, especially for high-risk patients hospitalized in hematology and bone marrow transplant units. Traditional monitoring involves impacted culture media that are incubated for several days so that the fungal species may grow and be identified on the basis of macroscopic and microscopic criteria. These systems are limited by their link with culture techniques, involving a time-lapse to results of around 7 days and by a maximal sampling rate of 100 L/min.
    This study was carried out to test the Coriolis combined with QPCR to assess its performance for detecting A. fumigatus DNA under 2 days in hematology units and in non-hematology hospital areas [1].

    Results

    The 14 air samples from hematology units were negative for A. fumigatus with both techniques (culture and PCR).
    Nine of the 19 air samples from non-hematology hospital areas were QPCR positive for A. fumigatus after sampling using the Coriolis μ with a mean and standard deviation of Cq of 39.38 ±1.09 and ten of the 19 air samples from non-hematology hospital areas were culture positive for A. fumigatus after impaction with the MAS 100 with a mean and standard deviation of 2.11±3.13 CFU/100 L of air (Table 1).

    Aspergillus QPCR Hospital 1

    In this experiment, a good concordance was observed, in terms of positive and negative results for the detection of A. fumigatus, between the two devices.
    Although the number of samples of this preliminary assay was limited, the Coriolis μ seems promising and could be an alternative for routine monitoring of fungal aero-contamination.

    Aspergillus QPCR Hospital 2

    [1] A.P Bellanger and al (2012): Contribution of a cyclonic based liquid air collector for detecting Aspergillus Fumigatus by QPCR in Air Samples. Journal of occupationnal and Environmental Hygiene, 9:1, D7-D11

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