Development of Ultra Rapid Assay System for Multiple Detection of Air borne Fungi by Coriolis Micro and multiplex pcr based dna Microarray - Bertin Instruments

    Research and Development

    Development of Ultra Rapid Assay System for Multiple Detection of Air borne Fungi by Coriolis Micro and multiplex pcr based dna Microarray

    Sources: Takayoshi Ootsu, Atsunori Isshiki Toyo Seikan Group Holdings, Ltd., Corporate Research & Development, Yokohama, Japan

    Context

    Air-borne fungi can cause biodeterioration and health problems. To avoid these damages, it is important to check the mycoflora in indoor environments such as factories, museums and other public facilities. Yet conventional methods for checking fungi need a cultivation process step.
    Studies [1] have reported that the Coriolis® μ concentration capability was enough to detect single fungal species by PCR amplification without cultivation process.
    In our study [2], we evaluated a ultrarapid assay system for multiplex detection of air-borne fungi composed of the multiplex PCR-based DNA microarray detection system GENOGATE [3] and the Coriolis® μ.

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