Fungal Assessment by culture and RT-PCR assay in a Waste Sorting Plant - Bertin Instruments

    Pollution and Environment

    Fungal Assessment by culture and RT-PCR assay in a Waste Sorting Plant

    Sources: Polytechnic Institute of Lisbon, Lisbon, Portugal

    Context

    In the management of solid waste, pollutants over a wide range are released with different routes of exposure for workers. The potential for synergism among the pollutants raises concerns about potential adverse health effects, and there are still many uncertainties involved in exposure assessment.
    In this study [1], molecular real-time polymerase chain reaction (RT-PCR) was used to assess the presence of three potential pathogenic/toxigenic fungal species: Aspergillus flavus, A. fumigatus, and Stachybotrys chartarum from air samples collected with a Coriolis µ in a waste-sorting plant. These results were compared with the cultivable fungal air contamination obtained by conventional method (culture-based).

    Results

    Penicillium sp. were the most common species found at all plant locations by conventional methodology. Pathogenic/toxigenic species (A. fumigatus and S. chartarum) were detected at two different workstations by RT-PCR but not by culture-based techniques (Table 1).

    fungi pcr waste
    Table 1: Characterization of the airbone fungi detected
     

    Our results illustrated the advantage of combining both conventional and molecular methodologies in fungal exposure assessment.
    Together with microbial volatile organic compounds (MVOC) analyses in indoor air, data obtained allow for a more precise evaluation of potential health risks associated with bioaerosol exposure. Consequently, with this knowledge, strategies may be developed for effective protection of the workers.
     

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