Sampling of P. jiroveciiin the Surrounding Air of Patients with Pneumocystis Pneumonia - Bertin Instruments

    Biomedical & Health

    Sampling of P. jiroveciiin the Surrounding Air of Patients with Pneumocystis Pneumonia

    Sources: Saint Louis Hospital, APHP, France

    Context

    Pneumocystis jirovecii is  responsible  for  a  disease  affecting  immuno-depressed  patients:  Pneumocystis pneumonia (PCP).  Airborne  transmission  of  Pneumocystis has  been  demonstrated  in  animal  models  and  is highly  probable  in  humans.  However,  information  concerning  burdens  of  Pneumocystis jirovecii in  exhaled  air  from infected patients is lacking due to the technical limitations of the current sampling methods. This fungus  is indeed not cultivable, so impaction method based on the cultivability of microorganisms cannot be used. Also, the difficulties of extraction from a solid support or the lengthy conditions of sampling (filtration technique) led us to consider alternative methods that would be suitable for both sampling and quantifying P. jirovecii in air. Our objective is to evaluate P. jirovecii air diffusion in patients with Pneumocystis pneumonia with Coriolis air sampler sampling combined with RT-PCR assays [1].

    Results

    33/56  positive  air  samples  :  the  more  the  distance  from  the

    patient is low, the more the P.jirovecii DNA is detected at:

    1m  – 15/19  samples  (78.9%),  3m  – 9/13  (69.2%),  5m  – 5/12

    (41.7%), 8m – 4/12 (33.3%)

    This  study  provides  the  first  quantitative  data  on  the

    spread of P. jirovecii in exhaled air from infected patients.

     

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