Over the past decades, to counteract the use of cannabis as a recreative drug, most world authorities have banned all Cannabis varieties without making a distinction. However, recently, some countries have authorized the cultivation of hemp (Cannabis sativa) cultivars characterized by a low amount of THC (tetrahydrocannabinol), responsible for Cannabis psychotropic effect. Since then, the benefits of hemp have attracted widespread attention in the food and wellness industry.

Consequently, an increasing number of food producers have started using hemp as an ingredient. To ensure consumer safety, reliable methods to verify and quantify the presence of Cannabis sativa will be needed.

In this application note, discover how DNA extracted from hemp seeds with the Minilys homogenizer was used to determine the optimal primer and probe concentrations for real-time PCR method.

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