The trypan blue dye is a commonly used staining agent to distinguish death tissues and cells from live cells. The method is well known and is widely used by a majority of research laboratories that conduct cell biology-based assays. Live cell counting is routinely performed manually using a microscope and a hemacytometer. However, accuracy and reliability of manual approach is limited, in addition to being a time consuming process.
Here, we report on how the use of a Total Cell Counting App available on InCellis®, rapidly and accurately number of dead and live cells in an in-vitro cell culture can be calculated in a novel automated approach.