Accurate & rapid viable cell counting in an automated manner using the InCellis® - Bertin Instruments

    Drug Screening & cell toxicity

    Accurate & rapid viable cell counting in an automated manner using the InCellis®

    Bertin Technologies & Team of Laboratory of Génétique et Biologie Cellulaire, EA 4589. University of Versailles St-Quentin-en-Yvelines, France

    Context

    The trypan blue dye is a commonly used staining agent to distinguish death tissues and cells from live cells. The method is well known and is widely used by a majority of research laboratories that conduct cell biology-based assays. Live cell counting is routinely performed manually using a microscope and a hemacytometer. However, accuracy and reliability of manual approach is limited, in addition to being a time consuming process.

    Here, we report on how the use of a Total Cell Counting App available on InCellis®, rapidly and accurately number of dead and live cells in an in-vitro cell culture can be calculated in a novel automated approach.

    Results

    Cell concentration can be calculated as follow:

    Cell concentration/ml = 2 × total cell number / 20 * 1000

    Cell concentration of 2.5 × 10e6 cells/ml was obtained in this study.

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