Automatic calculation of transfection efficiency with HELA cell culture - Bertin Instruments

    Gene Therapy

    Automatic calculation of transfection efficiency with HELA cell culture

    Bertin Technologies, Montigny-le-Bretonneux, France

    Context

    HeLa cell line is the oldest and most commonly used human cell line to analyze gene expression. Cell biologists need to generate cell images in living conditions with a high level of sensitivity to detect fluorescent labeling before working on high end complex microscopy platform.

    Transfection is a powerful tool to enabling study of the gene’s functions and gene products in cells. It’s important for biologists to check the transfection efficiency before performing further long and expensive experiments.

    InCellis® from Bertin Technologies is used to observe the cells and calculate the transfection efficiency thanks to the specific and dedicated application “Cell Transfection Efficiency”.

    Results

    Results

    Results

    Results

    Fig1: image in Phase Contrast, GFP and the overlay of HeLa Cell 48 hours after transfection.
    The transfection efficiency is 28%.
    With the kind participation of Pasteur Institute, Paris FRANCE.

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