Single cell based assays are powerful tools for studying drug effects, protein interactions and signaling pathways. However, the assay analysis depends on several factors that can impact the results. Obtaining an accurate number of cells within the cell culture and/or calculating cell culture confluency are some of the important factors that need to be taken into consideration prior the optimization of the results.
Total Cell counting is an efficient and simple cell culture InCellis App (InCellis® Smart Cell Imaging System (Bertin Technologies)), for adherent cell counting. In this note we list recommendations to generate accurate and robust results.