Centrosome is an organelle implicated in cell cycle regulation. During cell division, the two centrosomes serve as anchors to guide the chromosomes and divide them into two equitable batches to form the two daughter cells. An aberrant number of centrosomes in a cell is often associated with cancer.
Expression of GFP-centrin recombinant protein into in vitro cell culture allows direct visualization of centriole behavior in living cells in different experimental conditions. Nevertheless, as it is a small organelle, imaging of GFP-centrin protein into centrosome requires high sensitivity in fluorescence.
InCellis®, thanks to its low light sensor provides high quality images to easily control, directly on the bench, the quality of centrosome labeling before the analysis with a high end microscope.
A. Image in Phase contrast to control cell growth
B. Image of GFP labelling to control the stable transfection of Centrin – visualization of the centrosome (B’)
C. Image RFP labelling to control the stable transfection of Nucleolin – visualization of the nucleolus (C’)