Cell labelling qualification with the InCellis before using a high end microscope - Bertin Instruments

    Cell labelling qualification with the InCellis before using a high end microscope

    Bertin Technologies & UMR INSERM 1052 CNRS 5286, Lyon, France

    Context

    Centrosome is an organelle implicated in cell cycle regulation. During cell division, the two centrosomes serve as anchors to guide the chromosomes and divide them into two equitable batches to form the two daughter cells. An aberrant number of centrosomes in a cell is often associated with cancer.

    The expression of GFP-centrin recombinant protein into in vitro cell culture ensures direct visualization of centriole behavior in living cells in different experimental conditions. Nevertheless, as it is a small organelle, imaging GFP-centrin protein into centrosome requires high sensitivity in fluorescence.

    InCellis®, thanks to its low light sensor provides high quality images to easily control the quality of centrosome labeling directly on the bench, before analysis with a high-end microscope.

    Results

    Fig1:
    A. Image in Phase contrast to control cell growth
    B. Image of GFP labelling to control the stable transfection of Centrin – visualization of the centrosome (B’)
    C. Image RFP labelling to control the stable transfection of Nucleolin – visualization of the nucleolus (C’)

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