Since its launch in 2020, the Precellys Emulsion kit has been increasingly used to generate injectable antigen or antibody emulsions for research on auto-immune diseases. Discover the Top 2 open source research papers that Floriane Cohen, our Precellys Field Application Scientist, has selected for the optimization of the emulsion preparation protocols.
Standardization of Antigen-Emulsion Preparations for the Induction of Autoimmune Disease Models
Louise M. Topping, Laura Romero-Castillo, Vilma Urbonaviciute, Hans Bolinsson, Felix I. Clanchy, Rikard Holmdahl, B. Thomas Bäckström, and Richard O. Williams
Autoimmune disease models play an essential role in our understanding of a wide range of pathologies, including multiple sclerosis and rheumatoid arthritis. For most disease models, an antigen/adjuvant emulsion is used to induce disease. The quality of the emulsion is critical to ensure a potent immune response. In this article, the use of the Precellys Emulsion kit (Bertin Technologies, Montigny-les-Bretonneux, France) is validated for emulsion preparation in the context of experimental autoimmune encephalitis, collagen II induced arthritis, antigen induced arthritis, and delayed type hypersensitivity models. Results show that emulsions prepared with the Precellys Emulsion kits consistently and reproducibly induced diseases in the aforementioned models, and in some cases outperformed the traditional manual emulsion preparation method.
Mapping of neuroinflammation-induced hypoxia in the spinal cord using optoacoustic imaging
Marta Ramos-Vega, Pontus Kjellman, Mihail Ivilinov Todorov, Tekla Maria Kylkilahti, B. Thomas Bäckström, Ali Ertürk, Chris Denis Madsen & Iben Lundgaard
Recently, hypoxia and metabolic changes have been identified as potentially major actors in the pathophysiology of multiple sclerosis. In this study, researchers use optoacoustic imaging to analyze the changes in oxygenation and the vascular perfusion of the spinal cord in EAE (experimental autoimmune encephalomyelitis) a commonly used rodent model of multiple sclerosis. The Precellys Emulsion kit (Bertin Technologies, Montigny-les-Bretonneux, France is used to prepare Complete Freund’s Adjuvant (CFA)/antigen emulsions, which are later used to induce disease in mice. Results indicate a significant degree of hypoxia in EAE mice in vivo, as well as changes in spinal vascular perfusion.