This research focuses on the immune response during lung inflammation and/or infection in vivo. This requires isolating viable cells from lungs and characterizing the immune cell populations by flow cytometry.
Our standard protocol for isolating viable cells from lung tissue involves creating a single cell suspension by passing the tissue through a 70 μm cell sieve, which, when working with multiple samples, is highly time-consuming. Here we describe a novel rapid protocol for the isolation of immune cells from mice lungs using the Precellys Evolution tissue homogenizer. The optimized conditions allow the Precellys® Evolution, combined with the dedicated lysing kit, to be utilized for the isolation of viable immune cells from murine lungs that are suitable for phenotypic characterization.

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