The Precellys lysing kits are dedicated to sample preparation for Precellys tissue homogenizers. They gather high quality material (glass, ceramic or stainless steel) and Dnase/Rnase free certification.
Efficient and high-quality homogenization of the tissues is essential before analyzing the molecules of interest. Precellys Lysing Kits are specifically designed by Bertin Instruments to be used with Precellys tissue homogenizers. After optimized tissue lysis, the extraction of nucleic acids (DNA extraction, RNA extraction) is perfected using the DNA- and RNA-free guarantee of Precellys lysing kits.
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Get a quotePrecellys lysing kits are made of ceramic, glass, stainless steel or garnet, and are fabricated from high-quality materials. This, along with the different tube sizes available (0.3, 0.5, 2, 7 and 15 ml), provides great versatility for tissue homogenization. Over all, Bertin Instruments offers more than 30 different lysing matrices! The variety of Precellys lysing kits means that there is always a suitable solution for the homogenization of human, animal and plant tissues as well as microorganisms. Therefore, optimized homogenization reduces the processing time of the samples and consequently preserves the molecules of interest as much as possible while also ensuring an optimal recovered quantity. Choosing the right lysing kit for the tissue to be ground also limits the heating of the sample.
Choosing the right lysing kit for the tissue to be ground also limits the heating of the sample. Some types of samples require high energy grinding. This is the case for hair, bones, or plant seeds for example. In this context, the use of metal tubes ensures maximum tube strength in order to homogenize the samples as well as possible. In order to optimize the homogenization process of a given tissue, the Bertin Instruments Application Center helps you find the suitable protocol.
During the tissue homogenization, the initial inter- and intracellular compartmentalization is disrupted, releasing the enzymes directly in contact with their substrate. Proteases, which are usually responsible for protein degradation in the homogenized sample, are inhibited in Precellys Protein Safe Lysing Kits. The innovation of Bertin Instruments preserves the proteins in the samples and optimizes protein extraction to analyze them.
Protein Safe Soft tissue homogenizing CK14 – 2 mL
Includes 50 preps of 1.4mm ceramic (zirconium oxide) beads and Protein Safe solution in 2mL standard tubes. CK14 Protein Safe 2mL is designed for soft tissue homogenization while ensuring inhibition of endogenous proteases activity. Sample size range from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000973-LYSK0-A.0
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Soft tissue homogenizing CK14 – 0.5 mL
Includes 50 preps of 1.4mm ceramic (zirconium oxide) beads in 0.5mL standard tubes. CK14 0.5mL is designed for soft tissue homogenization like brain, liver, kidney, artery, and more. Sample size range: from 1mg to 20mg tissue or 5µL to 200µL culture.
Cat. No.: P000933-LYSK0-A
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Hard tissue homogenizing CK28 – 2 mL
Includes 50 preps of 2.8mm ceramic (zirconium oxide) beads in 2mL standard tubes. CK28 is designed for hard tissue homogenization like ear, lung, heart, spleen, tail, artery, Arabidopsis, leaves and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000911-LYSK0-A
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Soft tissue homogenizing CK14 – 2 mL
Includes 50 preps of 1.4mm ceramic (zirconium oxide) beads in 2mL standard tubes. CK14 is designed for soft tissue homogenization like brain, liver, kidney, skin, plant leaves, mammalian cells and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Now available with Protein safe tubes option!
Cat. No.: P000912-LYSK0-A
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Hard tissue homogenizing CK28-R – 2 mL
Includes 50 preps of 2.8mm ceramic (zirconium oxide) beads in 2mL reinforced tubes. Custom design reinforced tubes for bead beating application: O’ring and reinforced materials for dry grinding, valuable or very hard samples. CK28-R is designed for hard tissue homogenization like ear, lung, heart, spleen, tail, cornea, artery, Arabidopsis, leaves, tumor, intestine, adipose tissue and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Now available with Protein safe tubes option!
Cat. No.: P000916-LYSK0-A
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Tissue homogenizing CKMix – 2 mL
Includes 50 preps of ceramic (zirconium oxide) mix beads of 1.4mm and 2.8mm in 2mL standard tubes. CKMix is designed for tissue homogenization like brain, liver, kidney, ear, lung, heart, spleen, tail, artery, Arabidopsis, leaves, tumor, intestine, adipose tissue and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000918-LYSK0-A
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Tissue homogenizing CK28 – 7 mL
Includes 50 preps of 1.4mm ceramic (zirconium oxide) beads in 0.5mL standard tubes. CK14 0.5mL is designed for soft tissue homogenization like brain, liver, kidney, artery, and more. Sample size range: from 1mg to 20mg tissue or 5µL to 200µL culture. Cat. No.: KT03961-1-203.05 Includes 50 preps of 2.8mm ceramic (zirconium oxide) beads in 7mL standard tubes with O-ring. CK28 7mL is designed for tissue homogenization like whole rat brain, whole liver, kidney, spleen, leaves and more. Sample size range: from 200mg to 2g tissue or 200µL to 6mL culture.
Cat. No.: P000935-LYSK0-A
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Soft tissue homogenizing CK14 – 7 mL
Includes 50 preps of 1.4mm ceramic (zirconium oxide) beads in 7mL standard tubes with O-ring. CK14 7mL is designed for soft tissue homogenization like rat cerebellum, skin and more. Sample size range: from 200mg to 2g tissue or 200µL to 6mL culture.
Cat. No.: P000940-LYSK0-A
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Hard tissue homogenizing CK28 – 15 mL
Includes 25 preps of 2.8mm ceramic (zirconium oxide) beads in 15mL standard tubes. CK28 is designed for hard tissue homogenization like ear, lung, heart, spleen, tail, artery, Arabidopsis, leaves and more. Sample size range: from 400mg to 4g tissue or 400µL to 9mL culture.
Cat. No.: P000947-LYSK0-A
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Soft tissue homogenizing CK14_Well-Plate
Includes 12 x 8 tube stripes with individual cap organized in a 96 well-plate format (96×250µL) and containing 1.4mm ceramic (zirconium oxide) beads in each well. CK14 is designed for tissue homogenization like: mouse/rat tails, plants and small pieces of organs like: brain, liver, kidney, ear, lung, heart, spleen, artery, Arabidopsis, leaves, tumor, intestine, adipose tissue and more. Sample size range: from 0.6mg to 12mg tissue or 3µL to 120µL buffer.
Cat. No.: P002137-LYSK0-A
Protein Safe Hard tissue homogenizing CK28-R – 2 mL
Includes 50 preps of 2.8mm ceramic (zirconium oxide) beads and Protein Safe solution in 2mL reinforced tubes. CK28-R 2mL Protein Safe reinforced tubes include O’ring and reinforced materials for dry grinding, valuable or very hard samples while ensuring inhibition of endogenous proteases activity . Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000972-LYSK0-A.0
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Hard tissue grinding MK28 – 2 mL
Includes 50 preps of 2.8mm stainless steel beads in 2mL standard tubes. MK28 is designed for hard tissue grinding like muscle, skin, spinal cord, bone, teeth, hair, seeds, corn, rice and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000910-LYSK0-A
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Soil grinding SK38 – 2 mL
Includes 50 preps of a mix of glass and ceramic (zirconium oxide) beads in 2mL standard tubes. SK38 is designed for micro-organism lysing included in samples like soil, sediment, roots, faeces, animal or plant tissues and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000915-LYSK0-A
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Hard tissue grinding MK28-R – 2 mL
Includes 50 preps of 2.8mm stainless steel beads in 2mL reinforced tubes. Custom design reinforced tubes for bead beating application: O’ring and reinforced materials for dry grinding, valuable or very hard samples. MK28-R is designed for hard tissue grinding like muscle, skin, spinal cord, bone, teeth, hair, seeds, corn, rice and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000917-LYSK0-A
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Tissue grinding CKMix50-R – 2 mL
Includes 50 preps of ceramic (zirconium oxide) mix beads of 2.8mm and 5.0mm in 2mL reinforced tubes. Custom design reinforced tubes for bead beating application: O’ring and reinforced materials for dry grinding, valuable or very hard samples. CKMix50-R is designed for wet or dry tissue grinding like hair, whole piece of mouse lung, heart or dry hair, mouse bones and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000922-LYSK0-A
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Dry hard tissue grinding CK68-R – 2 mL
Includes 50 preps of ceramic (zirconium oxide) beads of 6.8mm in 2mL reinforced tubes. Custom design reinforced tubes for bead beating application: O’ring and reinforced materials for dry grinding, valuable or very hard samples. CK68-R is designed for dry samples grinding like rice grains, pepper grains and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000923-LYSK0-A
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Sample grinding GK60 – 2 mL
Includes 50 preps of garnet flakes 0.7mm and one ceramic (Zirconium Oxide, Ceria stabilized) in 2mL reinforced tubes. GK60 is designed for elastic tissues like skin, cartilage, retina and also general tissues like liver, plants and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000924-LYSK0-A
Soil Grinding SK38 – 7 mL
Includes 50 preps of a mix of ceramic (zirconium oxide) and glass beads in 7mL standard tubes with O-ring. SK38_7mL is designed for micro-organism lysing included in samples like soil, sediment, root, faeces, animal or plant tissues and more. Sample size range: from 200mg to 2g tissue or 200µL to 6mL culture.
Cat. No.: P000936-LYSK0-A
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Tissue grinding CKMix50 – 7 mL
Includes 50 preps of a mix of ceramic (zirconium oxide) mix beads of 2.8mm and 5.0mm in 7mL standard tubes with O-ring. CKMix50 7mL is designed for grinding whole organs like rat brain, liver, kidney, skin, whole iris-ciliary body pork and dry samples as pepper grains and more. Sample size range: from 200mg to 2g tissue or 200µL to 6mL culture.
Cat. No.: P000939-LYSK0-A
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Dry hard tissue grinding CK68 – 15 mL
Includes 25 preps of ceramic (zirconium oxide) beads of 6.8mm in 15mL standard tubes. CK68 is designed for dry samples grinding like rice grains, pepper grains and more. Sample size range: from 400mg to 4g tissue or 400µL to 9mL culture.
Cat. No.: P000950-LYSK0-A
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Tissue grinding CKMix50 – 15 mL
Includes 25 preps of a mix of ceramic (zirconium oxide) beads of 2.8mm and 5.0mm in 15mL standard tubes with O-ring. CKMix50_15mL is designed for grinding whole organs like rat brain, liver, kidney, skin, whole iris-ciliary body pork and dry samples as pepper grains and more. Sample size range: from 400mg to 4g tissue or 400µL to 9mL culture.
Cat. No.: P000949-LYSK0-A
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Metal tubes 7mL (x6)
Metal tubes for dry grinding of hard samples; only compatible with Precellys Evolution. Includes 6 stainless steel tubes of 7mL, one specific tube holder and one blocking plate compatible with Precellys Evolution. The Metal tubes are designed for dry grinding of very hard samples like: bones, tooth, hair, corn, and more. To be combined with beads in bulk. Sample size range: from 200mg to 1g;
Cat. No.: P000951-LYSK0-A
Metal tubes 2 mL
Stainless steel metal tubes for dry grinding of hard samples; only compatible with Precellys Evolution. Compatible with the tube holder of Plastic tubes 2 mL. The Metal tubes are designed for dry grinding of very hard samples like: bones, tooth, hair, corn, and more. To be combined with beads in bulk. Sample size range: from 20mg to 200mg.
Cat. No.: P002290-LYSK0-A.0
Tough micro-organism lysing VK05
Includes 12 strips of 8 tubes with individual caps arranged on a 96-well plate (96 × 250µL) and containing 0.5mm of glass beads each. VK05 is designed for the homogenization of microorganisms: bacteria, yeast, etc. Sample sizes: from 3 to 120µL
Cat. No.: P002136-LYSK0-A
Tough micro-organism lysing VK05 – 0.5 mL
Includes 50 preps of 0.5mm glass beads in 0.5mL standard tubes. VK05 0.5mL is designed for tough micro-organism lysing like yeast, fungi and more. Sample size range: from 1mg to 20mg sample or 5µL to 200µL culture.
Cat. No.: P000934-LYSK0-A
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Tough micro-organism lysing VK05 – 2 mL
Includes 50 preps of 0.5mm glass beads in 2mL standard tubes. VK05 is designed for tough micro-organism lysing like yeast, fungi, cyanobacteria, phytoplankton, nematode, spores, micro-algae and more. Sample size range: from 20mg to 200mg sample or 200µL to 1.6mL culture.
Cat. No.: P000913-LYSK0-A
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Tough micro-organism lysing VK05 – 15 mL – Certified DNase/RNase free
Includes 25 preps of 0.5mm glass beads in 15mL standard tubes. VK05 is designed for tough micro-organism lysing like yeast, fungi, cyanobacteria, phytoplankton, nematode, spores, micro-algae and more. Sample size range: from 400mg to 4g sample or 400µL to 9mL culture.
Cat. No.: P000961-LYSK0-A
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Micro-organism lysing VK01 – 2 mL
Includes 50 preps of 0.1mm glass beads in 2mL standard tubes. VK01 is designed for micro-organism lysing like gram-, gram+ bacteria, spores and more. Sample size range: from 20mg to 200mg sample or 200µL to 1.6mL culture.
Cat. No.: P000914-LYSK0-A
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Soil grinding SK38 – 2 mL
Includes 50 preps of a mix of glass and ceramic (zirconium oxide) beads in 2mL standard tubes. SK38 is designed for micro-organism lysing included in samples like soil, sediment, roots, faeces, animal or plant tissues and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000915-LYSK0-A
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Bacteria lysing CK01 – 2 mL
Includes 50 preps of 0.1mm ceramic (zirconium oxide) beads in 2mL standard tubes. CK01 is designed for micro-organism lysing like, gram+, gram- bacteria, spores and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000919-LYSK0-A
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Micro-organism lysing – VKMix – 7 mL
Includes 50 preps of glass mix beads of 0.1mm and 0.5mm in 7mL standard tubes with O-ring. VKMix 7mL is designed for micro-organism lysing like yeast fungi, gram+, gram- bacteria, spores, micro-algae and more. Sample size range: from 200mg to 2g sample or 200µL to 6mL culture.
Cat. No.: P000937-LYSK0-A
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Bacteria lysing CK01 – 7 mL
Includes 50 preps of 0.1mm ceramic (zirconium oxide) beads in 7mL standard tubes with O-ring. CK01 7mL is designed for micro-organism lysing like gram+, gram- bacteria, spores and more. Sample size range: from 200mg to 2g sample or 200µL to 6mL culture.
Cat. No.: P000938-LYSK0-A
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Soil Grinding SK38 – 7 mL
Includes 50 preps of a mix of ceramic (zirconium oxide) and glass beads in 7mL standard tubes with O-ring. SK38 7mL is designed for micro-organism lysing included in samples like soil, sediment, root, faeces, animal or plant tissues and more. Sample size range: from 200mg to 2g tissue or 200µL to 6mL culture.
Cat. No.: P000936-LYSK0-A
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Bacteria lysing CK01 – 15 mL
Includes 25 preps of 0.1mm ceramic (zirconium oxide) beads in 15mL standard tubes with O-ring. CK01_15mL is designed for micro-organism lysing like gram+, gram- bacteria, spores and more. Sample size range: from 400mg to 4g sample or 400µL to 9mL culture.
Cat. No.: P000948-LYSK0-A
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Soil grinding SK38 – 2 mL
Includes 50 preps of a mix of glass and ceramic (zirconium oxide) beads in 2mL standard tubes. SK38 is designed for micro-organism lysing included in samples like soil, sediment, roots, faeces, animal or plant tissues and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000915-LYSK0-A
Download the MSDS document
Tissue grinding CKMix50-R – 2 mL
Includes 50 preps of ceramic (zirconium oxide) mix beads of 2.8mm and 5.0mm in 2mL reinforced tubes. Custom design reinforced tubes for bead beating application: O’ring and reinforced materials for dry grinding, valuable or very hard samples. CKMix50-R is designed for wet or dry tissue grinding like hair, whole piece of mouse lung, heart or dry hair, mouse bones and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000922-LYSK0-A
Download the MSDS document
Sample grinding GK60 – 2 mL
Includes 50 preps of garnet flakes 0.7mm and one ceramic (Zirconium Oxide, Ceria stabilized) in 2mL reinforced tubes. GK60 is designed for elastic tissues like skin, cartilage, retina and also general tissues like liver, plants and more. Sample size range: from 20mg to 200mg tissue or 200µL to 1.6mL culture.
Cat. No.: P000924-LYSK0-A
Soil Grinding SK38 – 7 mL
Includes 50 preps of a mix of ceramic (zirconium oxide) and glass beads in 7mL standard tubes with O-ring. SK38 7mL is designed for micro-organism lysing included in samples like soil, sediment, root, faeces, animal or plant tissues and more. Sample size range: from 200mg to 2g tissue or 200µL to 6mL culture.
Cat. No.: P000936-LYSK0-A
Download the MSDS document
Tissue grinding CKMix50 – 7 mL
Includes 50 preps of a mix of ceramic (zirconium oxide) mix beads of 2.8mm and 5.0mm in 7mL standard tubes with O-ring. CKMix50 7mL is designed for grinding whole organs like rat brain, liver, kidney, skin, whole iris-ciliary body pork and dry samples as pepper grains and more. Sample size range: from 200mg to 2g tissue or 200µL to 6mL culture.
Cat. No.: P000939-LYSK0-A
Download the MSDS document
Tissue grinding CKMix50 – 15 mL
Includes 25 preps of a mix of ceramic (zirconium oxide) beads of 2.8mm and 5.0mm in 15mL standard tubes with O-ring. CKMix50_15mL is designed for grinding whole organs like rat brain, liver, kidney, skin, whole iris-ciliary body pork and dry samples as pepper grains and more. Sample size range: from 400mg to 4g tissue or 400µL to 9mL culture.
Cat. No.: P000949-LYSK0-A
Download the MSDS document
2 mL Empty Tubes and Caps with O-ring
Includes 500 units of 2mL tubes and caps with O-ring, non-assembled. Includes 500 units of 2mL tubes and caps with O-ring, disassembled. Empty 2mL tubes are designed for 2mL homogenization, grinding and lysing on Precellys Evolution, Precellys24, Minilys and Precellys24-Dual. Beads have to be added.
Cat. No.: P000945-LYSK0-A
2 mL Reinforced Empty Tubes and Caps
Includes 200 units of 2mL reinforced tubes and caps, assembled. Empty 2mL-Reinforced tubes are designed for 2mL-R homogenization, grinding and lysing on Precellys Evolution, Precellys24, Minilys and Precellys24-Dual. Beads have to be added.
Cat. No.: P000943-LYSK0-A
7 mL Empty Tubes and Caps
Includes 50 units of 7mL tubes and caps, assembled. Empty 7mL tubes are designed for 7mL homogenization, grinding and lysing on Precellys 24-Dual. Beads have to be added.
Cat. No.: P000944-LYSK0-A
15 mL Empty Tubes and Caps
Includes 50 units of 15mL tubes and caps, assembled. Empty 15mL tubes are designed for 15mL homogenization, grinding and lysing on Precellys Evolution. Beads have to be added.
Cat. No.: P000946-LYSK0-A
Precellys Emulsion Kit
Includes 10 autoclaved pouches including 1 Emulsion tube & cap and 1 specific Emulsion tube plunger
Support is provided online 24/7 by our Application Center, or by our dedicated Application Specialists
It allows users to homogenize, grind or lyse all sample types.
Pontifical Catholic University
Baylor College of Medicine
DNA extraction
DNA extraction
Obtaining high-quality, high-yield DNA from a sample requires an optimal homogenization process. Below, our team of scientists shares helpful tips to choose the right homogenization protocol for your DNA extraction
RNA extraction
RNA extraction
RNA isolation can often be challenging, particularly when working with complex samples. Common difficulties included low yield, low purity, RNA degradation, and DNA contamination. Below, Bertin’s team of scientists have shared best practices for homogenization protocols that allow for successful isolation of high-quality RNA.
Protein extraction
Protein extraction
Protein extraction is a key step for analytical methods such as Western Blot and enzymatic assays. Below, Bertin’s top scientists have shared best practices to improve your homogenization protocol for a wide range of tissues, including tips to preserve the integrity of the proteins in your samples and achieve optimal yield.
Choosing your lysing kit
Choosing your lysing kit
The disruption of samples is the first step in the process of isolation and/or quantification of nucleic acids, proteins, and analytes. For this reason, choosing the right homogenization protocol will have a great impact on the quality and reliability of results for most biological analytical workflows.
PCR and RT-PCR
PCR and RT-PCR
PCR is a vital analytical tool for modern biologists. The success of PCR workflows is highly dependent on the quantity, purity, and degree of fragmentation of the DNA. Here, Bertin’s top scientists have presented examples of optimized homogenization protocols to obtain high quality nucleic acids and guarantee the success of your PCR workflow.
NGS
NGS
The advent of next-generation sequencing techniques (NGS) has dramatically changed the field of biology. Below, Bertin’s team of scientists have introduced optimized sample preparation protocols examples to ensure high quality, high yield nucleic acids for your NGS workflows.
Metabolite extraction
Metabolite extraction
Successfully extracting metabolites is a crucial step in metabolite profiling. Discover here our top scientists’ homogenization protocols for metabolite extraction from a wide range of samples.
Drug extraction
Drug extraction
Drug extraction is a critical step in pharmaceutical, toxicological, environmental, and food analysis. On this page, our scientists have listed a selection of our best protocols for drug extraction from a wide range of tissues.
Mass spectrometry
Mass spectrometry
Mass spectrometry is an essential analytical technique in chemistry and biology. Below our experts share their advice on improving homogenization protocols for mass spectrometry workflows for protein, metabolites, and drug analysis in complex samples.
Western Blot
Western Blot
Western Blot is a widely used molecular biology technique to detect specific proteins in a sample. On this page, our experts present their tips and tricks to improve your homogenization protocol for Western Blot, as well as a chosen selection of our best application notes on Western Blot.
Chromatography
Chromatography
Chromatography is a laboratory technique to separate, identify, and quantify each component in a sample. Below, our tops experts have shared a selection of our best sample preparation protocols for chromatography workflows.
Environment
Soil and environmental analysis often focus on microbiology studies and pollution impact on wildlife. Microorganisms included in a biological matrix are particularly challenging to lyse.
Microbiology
Microorganism studies are critical as they are used for bio-production and health research. They are difficult to lyse due to their thick cell wall, which makes nucleic acid extraction difficult.
Pharmacology
Pharmaceutical research starts with lead molecule identification from a natural or artificial model. In either case, the DMPK or animal toxicology models analysis requires the homogenization of tissues.
Forensic
Extremely hard samples such as bone, teeth, and hair are common in forensic laboratories. Popular downstream applications include DNA extraction from bone or teeth and drug extraction from hair.
Agronomy
Agronomy research into GMO analysis, food allergies and plant studies, often require grounded hard samples such as seeds, roots or frozen food, which are particularly hard to grind.
Cancer research
Hard tissues such as those from tumors can be particularly difficult to homogenize, and animal models are often used in cancer research. Precellys homogenizer is perfectly adapted to applications of this kind.
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