Tissue Homogenizers – Sample preparation - Bertin Instruments
Sample preparation is critical to master molecules extraction from biological samples. Bertin Instruments offers efficient and flexible Precellys tissue homogenizers for grinding any samples prior to analysis.
In order to extract the molecules of interest (extraction of RNA, DNA, proteins, metabolites, etc.) from biological samples, sample preparation procedures generally include a tissue homogenization or cell disruption step.
The Precellys® homogenizers from Bertin Instruments use bead-beating technology to homogenize biological samples, such as human, animal or plant tissues, or to lyse micro-organisms. In this way, the biological samples are ground via multi-directional movement (3D bead-beating), the speed of which can reach up to 10,000 rotations per minute, depending on the equipment. Bead-beating is the gold standard method for tissue homogenization compared to standard techniques such as: mortar & pestle, sonication, blender, high pressure homogenizer. It takes just a few dozen seconds to homogenize the samples, which are then ready for an analysis of the molecules of interest (nucleic acids, proteins, metabolites) using different analysis techniques: PCR, sequencing, Western Blot, liquid chromatography, etc. This represents a considerable time saving that simplifies sample preparation protocols.
The high-performance characteristics of the Bertin Instruments homogenizers, together with a wide variety of lysing and grinding kits, offer excellent reproducibility and a great degree of flexibility in a broad range of research areas including microbiology, pharmacology, cancer, forensic medicine, the environment, and even agronomy.
The Precellys® range allows you to prepare samples in full safety and without cross contamination, for both routine applications and R&D projects.
• For infrequent use, the Minilys® homogenizer allows you to prepare up to three samples at the same time. Its flexibility (0.5, 2 and 7 ml tubes) and its small size make it ideal for laboratories needing conventional sample preparation.
• The Precellys® 24 homogenizer can accommodate up to 24 biological samples in 2 ml and 0.5 ml tubes simultaneously. This robust appliance has been designed for daily use in laboratories with biological safety level 3.
• For even greater versatility, the Precellys® Evolution can accommodate up to 96 samples at the same time with six different tube volumes (from 0.3 ml to 15 ml), and meets the safety standards of laboratories with biological safety level 3.Avoid degradation of your heat-sensitive samples during homogenization with Cryolys® Evolution, the sample cooling system compatible with Precellys®
DNA extraction
DNA extraction
Obtaining high-quality, high-yield DNA from a sample requires an optimal homogenization process. Below, our team of scientists shares helpful tips to choose the right homogenization protocol for your DNA extraction
RNA extraction
RNA extraction
RNA isolation can often be challenging, particularly when working with complex samples. Common difficulties included low yield, low purity, RNA degradation, and DNA contamination. Below, Bertin’s team of scientists have shared best practices for homogenization protocols that allow for successful isolation of high-quality RNA.
Protein extraction
Protein extraction
Protein extraction is a key step for analytical methods such as Western Blot and enzymatic assays. Below, Bertin’s top scientists have shared best practices to improve your homogenization protocol for a wide range of tissues, including tips to preserve the integrity of the proteins in your samples and achieve optimal yield.
Metabolite extraction
Metabolite extraction
Successfully extracting metabolites is a crucial step in metabolite profiling. Discover here our top scientists’ homogenization protocols for metabolite extraction from a wide range of samples.
Drug extraction
Drug extraction
Drug extraction is a critical step in pharmaceutical, toxicological, environmental, and food analysis. On this page, our scientists have listed a selection of our best protocols for drug extraction from a wide range of tissues.
Choosing your lysing kit
Choosing your lysing kit
The disruption of samples is the first step in the process of isolation and/or quantification of nucleic acids, proteins, and analytes. For this reason, choosing the right homogenization protocol will have a great impact on the quality and reliability of results for most biological analytical workflows.
PCR and RT-PCR
PCR and RT-PCR
PCR is a vital analytical tool for modern biologists. The success of PCR workflows is highly dependent on the quantity, purity, and degree of fragmentation of the DNA. Here, Bertin’s top scientists have presented examples of optimized homogenization protocols to obtain high quality nucleic acids and guarantee the success of your PCR workflow.
NGS
NGS
The advent of next-generation sequencing techniques (NGS) has dramatically changed the field of biology. Below, Bertin’s team of scientists have introduced optimized sample preparation protocols examples to ensure high quality, high yield nucleic acids for your NGS workflows.
Western Blot
Western Blot
Western Blot is a widely used molecular biology technique to detect specific proteins in a sample. On this page, our experts present their tips and tricks to improve your homogenization protocol for Western Blot, as well as a chosen selection of our best application notes on Western Blot.
Mass spectrometry
Mass spectrometry
Mass spectrometry is an essential analytical technique in chemistry and biology. Below our experts share their advice on improving homogenization protocols for mass spectrometry workflows for protein, metabolites, and drug analysis in complex samples.
Chromatography
Chromatography
Chromatography is a laboratory technique to separate, identify, and quantify each component in a sample. Below, our tops experts have shared a selection of our best sample preparation protocols for chromatography workflows.
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Cancer Research
Hard tissues such as those from tumors can be particularly difficult to homogenize, and animal models are often used in cancer research. Precellys homogenizer is perfectly adapted to applications of this kind.
Agronomy
Agronomy research into GMO analysis, food allergies and plant studies, often require grounded hard samples such as seeds, roots or frozen food, which are particularly hard to grind.
Forensic
Extremely hard samples such as bone, teeth, and hair are common in forensic laboratories. Popular downstream applications include DNA extraction from bone or teeth and drug extraction from hair.
Pharmacology
Pharmaceutical research starts with lead molecule identification from a natural or artificial model. In either case, the DMPK or animal toxicology models analysis requires the homogenization of tissues.
Environment
Soil and environmental analysis often focus on microbiology studies and pollution impact on wildlife. Microorganisms included in a biological matrix are particularly challenging to lyse.
Microbiology
Microorganism studies are critical as they are used for bio-production and health research. They are difficult to lyse due to their thick cell wall, which makes nucleic acid extraction difficult.
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