Bone Morphogenetic Protein 2 (BMP-2) is one of the most potent growth factors involved in bone growth and repair. Since it is present at low levels, its extraction and quantitation from bone is challenging. To evaluate the quantitative recovery of recombinant BMP-2 after a stringent grinding cycle in a Precellys® Evolution, we spiked commercial, demineralized cancellous bone with the protein, pulverized the samples, and measured the level of BMP-2 in the ground sample with an industry standard immunoassay.
The extract from the unspiked demineralized cancellous bone did not yield any signal in the Quantikine assay, indicating that its BMP-2 content was below the detection level of the assay.
For the extract of the spiked bone sample, the Quantikine assay measured a level of BMP-2 of 227 ng/ml, which corresponded to a 101% recovery of the BMP-2 spiked into the sample.