Assessment of Salmonella Typhimurium load within infected nematodes - Bertin Instruments

    Microbiology

    Assessment of Salmonella Typhimurium load within infected nematodes

    Sources: School of Biosciences, University of Birmingham,Birmingham, UK.

    Context

    The nematode Caenorhabditis elegans is a powerful model system for the study of host-pathogen interactions. This study looked at innate immune response of C.elegans to Salmonella Typhimurium. To test whether the altered resistance of lys-7 and abl-1(tyrosine kinase ABL-1) mutant nematodes to Salmonella Typhimurium was due to a lower bacterial load within infected animals, wild type (N2), lys-7 and abl-1 animals were exposed to S. Typhimurium L1019, and infectious burden quantified over time through viable counts[1].

     

    [1] Marsh EK, van den Berg MCW, May RC (2011) A Two-Gene Balance Regulates Salmonella TyphimuriumTolerance in the Nematode Caenorhabditis elegans. PLoS ONE 6(3): e16839.doi:10.1371/journal.pone.0016839

    Results

    Infection load was assessed through viable counts for 5 days / each 24 hours. lys-7 and abl-1 mutant animals are tolerant of S. Typhimurium infection (Figure 1a). There was no difference between any of the strains (p.0.2) showing that lys-7 and abl-1 single mutants are tolerant to S. Typhimurium infection for at least six days (Figure 1b).

     

    Figure 1: (a) S.Typhimurium load per nematode and (b) nematode survival curve.

    Figure 1: (a) S.Typhimurium load per nematode and (b) nematode survival curve.

     

    img-03712-810-DU060-2

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