Bacillus Cereus – Bacteria – Protein Extraction - Bertin Instruments

    Protocol

    Bacillus Cereus – Bacteria – Protein Extraction

    Sample type

    Bacteria

    Buffer

    1 mL of a lysis buffer consisting in 7.8 M Urea (Sigma), 2.2 M Thiourea (Sigma), 4.5% w/v CHAPS 9 (Sigma), 44.5 mM DTT (Sigma), 2.2 mM Trizma base (Sigma)/HCl (Sigma) pH 7.5, and one tablet of antiprotease mini-cocktail (Roche)

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