The Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that induces in humans a disease characterized by fever, rash, and pain in muscles and joints. The aim of this study is to propose a new model for CHIKV infection in adult, immunocompetent cynomolgus macaques. CHIKV infection in these animals recapitulated the viral, clinical, and pathological features observed in human disease .
 K.labadie et al, J Clin Invest. 2010;120(3):894–906. doi:10.1172/JCI40104. http://www.jci.org
The figure 1 shows the infectious virus titers in spleen, liver, muscle, and joint collected from CHIKV-infected macaques, 6 and 44 dpi.
Figure 1. Tissues were collected at 6 dpi from macaques inoculated i.v. with 107 PFU CHIKV, or at 44 dpi from macaques inoculated i.v. with 106 PFU CHIKV, and the amount of infectious virus present in tissues was quantified by TCID50. Data are mean ± SEM of 2 independent virus titrations. The detection threshold was 700 TCID50/g.
Our results provide insights into the pathogenesis of CHIKV. We have developed a relevant macaque model of CHIKV infection, in which we demonstrated long-term CHIKV persistence in various tissues and identified macrophages as cellular reservoirs during the late stages of CHIKV infection in vivo.