Development and field evaluation of a nested RT-PCR kit for detecting Japanese encephalitis virus in mosquitoes - Bertin Instruments

    Protocol

    Development and field evaluation of a nested RT-PCR kit for detecting Japanese encephalitis virus in mosquitoes

    Young Eui Jeong a, Min Ju Jeon a, Jung Eun Cho a, Myung Guk Han a, Hwan Ju Choi b, Mi Yeong Shin c, Hag Jae Park c, Woosik Kim d, Bong Chun Moon e, Ji-Sung Park f, Bona Park f and Young Ran Ju a
    Sources: a WHO Japanese Encephalitis Regional Reference Laboratory for the Western Pacific Region/Division of Arboviruses, National Institute of Health, Korea Centers for Disease Control and Prevention, 194 Tongil-lo, Eunpyung-gu, Seoul, 122-701, Republic of Korea b Gyeong-Nam Research Institute of Health and Environment,, Changwon-si, Gyeongsangnam-do, 641-702, Republic of Koreac Jeon-Nam Research Institute of Health and Environment,, Seo-gu, Gwangju, 502-200, Republic of Koread Chung-Nam Research Institute of Health and Environment,, Dong-gu, Daejeon, 300-801, Republic of Koreae Institute of Environment,al Resource Research of Jeju Special Self-Governing Province, Jeju city, Jeju-Do, 690-817, Republic of Koreaf Department of Manufacturing & Development, Intron Biotechnology, Inc., JungAng Induspia B/D, Gyeonggi-Do, 462-120, Republic of Korea
    Journal of Virological MethodsArticle in Press, Uncorrected Proof - Note to users doi:10.1016/j.jviromet.2010.11.009

    Sample type

    Animal tissue (insect), Virus

    Homogenizers

    1.5ml of sterile phosphate buffered saline (PBS)

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