Direct Quantitative Determination of a Nucleotide Diphosphate in Monkey and Rabbit Tissues using LC-MS/MS - Bertin Instruments

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Direct Quantitative Determination of a Nucleotide Diphosphate in Monkey and Rabbit Tissues using LC-MS/MS

Sources: Pyxant Labs, Inc., Colorado Springs, Colorado, USA

Context

A sensitive, accurate and precise bioanalytical method was developed for the determination of Cidofovir diphosphate in monkey and rabbit tissues using LCMS/ MS. The method was developed to evaluate concentration levels of the active metabolite in various monkey and rabbit organs and tissues in support of a maximum tolerated dose study [1].

 

[1] Foley S, Begley J, Karnik S, Keilholz L, Lambert B. DirectQuantitative Determination ofCidofovir Diphosphate in Monkey and Rabbit Tissues using LC/MSMS.2012 ASMS meetingabstracts.

Results

The Precellys®24 allowed validation of this method over a linear range of 5 to 1000 pg/mg. Full validations were performed for monkey and rabbit liver tissue. Validation experiments included intra- and inter-day precision and accuracy, selectivity/specificity, recovery, matrix effect, lower and upper limit of quantification and stability tests. The accuracy for monkey liver ranged from 97.7% to 101% and the precision ranged from 4.3% to 8.2% (CV). For rabbit liver, the accuracy range was 101% to 102% and the precision range was 4% to 7.5% (CV).

 

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