DNA extraction from Arabidopsis leaves using Precellys® Evolution vs Precellys®24-Dual - Bertin Instruments

    Environment

    DNA extraction from Arabidopsis leaves using Precellys® Evolution vs Precellys®24-Dual

    Sources: PEQLAB, Erlangen, Germany

    Context

    The new Precellys® Evolution is even more powerful (up to 10000 rpm) and versatile (0.5, 2.0, 7.0 and 15 ml tubes). Increased grinding power is beneficial for tough or stringy tissues. However, for soft tissues it is known that too much power can lead to degradation of target molecules like DNA. Therefore the goal was to compare DNA quality (integrity) and quantity (yield) after homogenization of Arabidopsis leaves by Precellys® Evolution or Precellys®24-Dual at given rpm.

    Results

    The gel picture obtained for Precellys® Evolution (Fig. 1) shows that the yield and quality is comparable in the range of 4600 – 8800 rpm, whereas degradation was observed at 10000 rpm.
    The gel picture obtained for Precellys® 24-Dual (Fig. 2) confirms that no degradation is observed up to 6500 rpm. Average yield was 2 μg DNA.

    gel-picture-obtained-for-precellys-evolution

    Figure 1: Agarose gel (1.5%, 1xTAE) electrophoresis of DNA isolated from Arabidopsis leaves homogenized with Precellys® Evolution at given rpm. M = DNA sizer II

    gel-picture-obtained-for-precellys24-dual

    Figure 2: Agarose gel (1.5%, 1xTAE) electrophoresis of DNA isolated from Arabidopsis leaves homogenized with Precellys® 24-Dual at given rpm. M = DNA sizer II

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