DNA extraction from bovine liver tissue using Precellys® Evolution vs Precellys®24-Dual - Bertin Instruments

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DNA extraction from bovine liver tissue using Precellys® Evolution vs Precellys®24-Dual

Sources: PEQLAB, Erlangen, Germany

Context

The new Precellys® Evolution is even more powerful (up to 10000 rpm) and versatile (0.5, 2.0, 7.0 and 15 ml tubes). Increased grinding power is beneficial for tough or stringy tissues. However, for soft tissues it is known that too much power can lead to degradation of target molecules like DNA.

Results

The gel picture obtained for Precellys® Evolution (Figure 1) shows that rpm values >7200 rpm lead to visible DNA degradation in bovine liver tissue. Yield from 4600 – 7200 rpm was 48 μg; higher energy reduced the yield to approximately 28 μg DNA.

The gel picture obtained for Precellys®24-Dual
(Figure 2) shows that no degradation is observed up to 6500 rpm. The average yield was 45 µg DNA

Image4

Figure 1: Agarose gel (1.5%, 1xTAE) electrophoresis of DNA isolated from bovine liver homogenized with Precellys® Evolution at given rpm. M = DNA sizer II

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Figure 2: Agarose gel (1.5%, 1xTAE) electrophoresis of DNA isolated from bovine liver homogenized with Precellys® 24-Dual at given rpm. M = DNA sizer II

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