Dna Extraction from Chlorella Sorokiniana (Algae) - Bertin Instruments

Environment

Dna Extraction from Chlorella Sorokiniana (Algae)

Context

Precellys®24 has been used by Promega internal application lab to extract gDNA from Chlorella sorokiniana, before sequencing (NGS). The efficiency of extraction with Precellys®24 has been compared to Proteinase K method based on heating treatment. DNA purification was performed using Promega Maxwell 16 equipment with the LEV Plant DNA kit.

Results

DNA extraction yields obtained with Precellys®24 or with the Proteinase K method have been evaluated by NanoDrop quantification.

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High  extraction  yields  are  obtained  with  Precellys ® 24 compared to the Proteinase K method. The ratios 260/280 and 260/230 are also very good with Precellys ® 24.

Thanks to the Precellys ® 24, high quality DNA  is extracted from Chlorella sorokiniana in high quantity.

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