Precellys®24 has been used by Promega internal application lab to extract gDNA from Chlorella sorokiniana, before sequencing (NGS). The efficiency of extraction with Precellys®24 has been compared to Proteinase K method based on heating treatment. DNA purification was performed using Promega Maxwell 16 equipment with the LEV Plant DNA kit.
DNA extraction yields obtained with Precellys®24 or with the Proteinase K method have been evaluated by NanoDrop quantification.
High extraction yields are obtained with Precellys ® 24 compared to the Proteinase K method. The ratios 260/280 and 260/230 are also very good with Precellys ® 24.
Thanks to the Precellys ® 24, high quality DNA is extracted from Chlorella sorokiniana in high quantity.