DNA extraction from fetal and neonatalpathology tissues - Bertin Instruments

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    DNA extraction from fetal and neonatalpathology tissues

    Sources: at the West Midlands Regional Genetics Laboratory, UK

    Context

    The West Midlands Regional Genetics Laboratory (WMRGL) is an NHS diagnostic laboratory providing testing for a wide range of genetic disorders. Fetal and perinatal pathology tissue samples are analyzed for chromosome abnormalities by QF-PCR and subtelomere MLPA. Prior to the introduction of the Precellys®24 these tissue samples were finely chopped using a scalpel, digested for >24 hours followed by a manual salting out DNA extraction procedure.

    Results

    DNA was extracted by Qiagen EZ1 robot using the tissue protocol. The results are reported in the figure 1.

     

    img-03712-810-DU064

    Concentration comparison of previous extraction method to Precellys®/EZ1 extraction.

    Agarose gel image of DNA extracted by Precellys 24 / EZ1 extraction. Samples showed no or minimal DNA degradation.

    Agarose gel image of DNA extracted by Precellys 24 / EZ1 extraction. Samples showed no or minimal DNA degradation.

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