DNA extraction from French soils Evaluation of Precellys®24-Dual 7mL Vial - Bertin Instruments

    Environment

    DNA extraction from French soils Evaluation of Precellys®24-Dual 7mL Vial

    Sources: Genosol – UMR Microbiology of the soil and environment,Inra Dijon, France

    Context

    The Genosol platform is a biological resource centre unique in France and Europe, devoted to the conservation and analysis of the genetic resources of soil microbial communities. The ambition of the GenoSol platform is to centralize the microbial genetic resources of soils and make them available to the scientific community together with the most upto-date technologies for their characterization. In this way, the Precellys®24 -Dual was evaluated in comparison with the reference method.

    Results

    With Precellys®24 – Dual, the concentration of DNA extracted is around 2.5 higher than the standard method. The quality of DNA, evaluated by inhibition and restrictive enzyme assays was equivalent with both equipments. Precellys®24 – Dual allows a better cadence: 6 samples could be prepared in 45s versus 120s with standard method (1 sample by run).

    DNA Extraction soil
    DNA concentration (ng/g soil) for 3 French soils.

     

    Gel electrophoresis analysis, Epoisses soil (triplicate).

    Gel electrophoresis analysis, Epoisses soil (triplicate).

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