DNA extraction from Oyster Larvae Crassostrea Gigas and Crassostrea Angulata - Bertin Instruments

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DNA extraction from Oyster Larvae Crassostrea Gigas and Crassostrea Angulata

Sources: IFREMER-Laboratoire de Génétique et Pathologie des Mollusques Marins-FRANCE

Context

This laboratory is working on the genetic diversity of cupped oysters at different scales. This study is focusing on hybridization and introgressive hybridization between the species C. gigas and C. angulata. Both, coming from Asia, are present in South Portugal in Europe. To characterize their relationship, F2 experimental crosses of biparental families were performed in our experimental hatchery. A panel of specific SNP will be used on each individual larvae. Therefore a grinding method is necessary to extract DNA. Our study was designed to see if the Precellys kit lysis permits to grind efficiently larvae to obtain homogenous lysis and good quality and quantity of DNA materials, with reproducibility, in order to perform PCR amplification.

Results

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DNA  extraction  and  quantification  performed on 5 larvae.

 

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Test with 16 S PCR amplification

 

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