The research plan is to isolate the genes of functional proteins and clarify their functions by introducing them into plants. The lab is studying the gene transfection into a plant seed by electroporation. Our team checks how much volume of DNA is introduced into a seed by a standard molecular biology process.
DNA was extracted with Nucleon™ PhytoPure™ Genomic DNA Extraction Kits from GE Helthcare. The migration gives the following results : (Lane No.1 and No.2 are replications). Since the concentration of the DNA is known, it is used to semi-quantify the amount of extracted DNA by visual observation.
Fresh leaves, before LN