DNA Extraction from Rice Leaf - Bertin Instruments

Agronomy

DNA Extraction from Rice Leaf

Sources: National Institute of Agrobiological Sciences - Japan

Context

The research plan is to isolate the genes of functional proteins and clarify their functions by introducing them into plants. The lab is studying the gene transfection into a plant seed by electroporation. Our team checks how much volume of DNA is introduced into a seed by a standard molecular biology process.

Results

DNA was extracted with Nucleon™ PhytoPure™ Genomic DNA Extraction Kits from GE Helthcare. The migration gives the following results : (Lane No.1 and No.2 are replications). Since the concentration of the DNA is known, it is used to semi-quantify the amount of extracted DNA by visual observation.

DNA extraction rice leaf

Fresh leaves, before LN

 

Frozen leaves after grinding

Frozen leaves after grinding

 

1. DNA from rice leaf 2. DNA from rice leaf 3. ?DNA 0.05µg 4. ?DNA 0.1µg 5. ?DNA 0.5µg 6. Marker 7. Marker

1. DNA from rice leaf 2. DNA from rice leaf 3. ?DNA 0.05µg 4. ?DNA 0.1µg 5. ?DNA 0.5µg 6. Marker 7. Marker

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