PharmOptima focuses in vivo and in vitro DM&PK and Discovery Services, providing IND & NDA-enabling research protocols. DM&PK with an ocular focus is a part of the laboratory’s routine research. PharmOptima lab currently uses Precellys to homogenize a variety of different animal tissues. In this study, Minilys and probe sonicator have been compared to current method (Precellys). Advantages & drawbacks are discussed.
The analyte levels by samples are equivalent. No difference involving the three homogenizing techniques are detectable (See Figure 1). Despite one sample prep in 7mL vial, Minilys is preferred rather the probe sonicator method which is fastidious and time consuming with cleaning steps and potential contamination between samples.
Figure 1: LC-MS/MS analysis from Precellys Dual, Minilys, and Sonicator sample.