Effective and automated pulverization of mouse muscle using the precellys evolution - Bertin Instruments

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Effective and automated pulverization of mouse muscle using the precellys evolution

Sources: Sanford-Burnham-Prebys Medical Discovery Institute, Orlando, Florida, USA

Context

Homogeneous, small particle powders are important to obtain the highest yield of metabolites extracted from tissues for metabolomics experiments. This is especially important when tissue powder from the same tissue needs to be aliquoted and homogenized in different extraction solvents to quantitate different classes of metabolites. The purpose of this study was to explore the utility of the Precellys Evolution to efficiently provide uniform, finely ground powders from difficult to grind tissues like mouse gastrocnemius compared to common methods like cryogrinding in a mortal and pestle.

Results

Different tissue particle sizes are obtained from manual cryogrinding compared to Precellys Evolution pulverization of lyophilized mouse gastrocnemius.

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Figure 1.  Manual cryogrinding of lyophlized mouse gastrocnemius produces a large, particle powder.

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Figure 2. Precellys Evolution pulverization of mouse gastrocnemius produces a fine particle powder.

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