HOMOGENIZATION AND EXTRACTION OF FLUORESCENT CHEMICAL COMPOUNDS FROM MOUSE BONE TISSUE - Bertin Instruments

Pharmacology

HOMOGENIZATION AND EXTRACTION OF FLUORESCENT CHEMICAL COMPOUNDS FROM MOUSE BONE TISSUE

Sources: Atlanthera Laboratories, Saint-Herblain, France

Context

Atlanthera is a drug discovery company specialized in bone-targeted drug delivery through covalent binding of drugs to hydroxybisphosphonates (HBP), a class of molecule known for high affinity to bone. Extraction of HBP-conjugated chemical from bone is challenging and require efficient tissue homogenization. Precellys® Evolution system was tested for bone homogenization and compared with a cryogenic grinder.

Results

The best homogenization efficiency was obtained at 8500 rpm on dry rather than homogenization in buffer.
Compound extraction was equivalent to our standard protocol (cryogenic grinder)

SPPP-810-DU124_Mouse bone

Figure 1: Example for HPLC analysis of compound extracted from bone tissue after homogenization with Precellys® Evolution

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