Atlanthera is a drug discovery company specialized in bone-targeted drug delivery through covalent binding of drugs to hydroxybisphosphonates (HBP), a class of molecule known for high affinity to bone. Extraction of HBP-conjugated chemical from bone is challenging and require efficient tissue homogenization. Precellys® Evolution system was tested for bone homogenization and compared with a cryogenic grinder.
The best homogenization efficiency was obtained at 8500 rpm on dry rather than homogenization in buffer.
Compound extraction was equivalent to our standard protocol (cryogenic grinder)
Figure 1: Example for HPLC analysis of compound extracted from bone tissue after homogenization with Precellys® Evolution