Homogenization of 6 brains of mouse in a 15mL Precellys® tube using the Precellys® Evolution - Bertin Instruments

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    Homogenization of 6 brains of mouse in a 15mL Precellys® tube using the Precellys® Evolution

    Sources: Bertin Pharma, Orléans, France

    Context

    The laboratory of Bertin Pharma works in the field of pharmacy, biotechnologies, cosmetics and nutrition (GLP & GMP). It is specialized in bioanalysis, formulation analysis, pesticide assays, and membrane protein quantification. In several applications, matrices are crushed before LC/MS/MS or LC-UV analysis. Different kinds of matrices are studied (brain, liver, muscle, spinal cord of rats, plants, pollens, vegetables, bees) and different systems are used for sample preparation (Ultra-Turrax, Precellys®24-Dual and Precellys® Evolution). The goal was to prepare a higher quality homogenate in less time.

    Results

    The brain homogenate obtained using Precellys® Evolution was judged equivalent as the one obtained using Precellys®24-Dual (Figure 1).

    Image1

    a) before homogenization

     

    Image2 Image3

    b) after homogenization

    The main goal was to prepare a higher quality homogenate in less time. 36 brains can be treated simultaneously (6 brains/15mL Precellys® tube x6) using Precellys® Evolution instead of 24 (4 brains/7mL Precellys® tube x 6) with Precellys®24-Dual. No difference was observed between the 2 evaluated programs.

     

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