The laboratory of Bertin Pharma works in the field of pharmacy, biotechnologies, cosmetics and nutrition (GLP & GMP). It is specialized in bioanalysis, formulation analysis, pesticide assays, and membrane protein quantification. In several applications, matrices are crushed before LC/MS/MS or LC-UV analysis. Different kinds of matrices are studied (brain, liver, muscle, spinal cord of rats, plants, pollens, vegetables, bees) and different systems are used for sample preparation (Ultra-Turrax, Precellys®24-Dual and Precellys® Evolution). The goal was to prepare a higher quality homogenate in less time.
The brain homogenate obtained using Precellys® Evolution was judged equivalent as the one obtained using Precellys®24-Dual (Figure 1).
a) before homogenization
b) after homogenization
The main goal was to prepare a higher quality homogenate in less time. 36 brains can be treated simultaneously (6 brains/15mL Precellys® tube x6) using Precellys® Evolution instead of 24 (4 brains/7mL Precellys® tube x 6) with Precellys®24-Dual. No difference was observed between the 2 evaluated programs.