Immunoaffinity mass spectrometry for leukotriene analysis in brain - Bertin Instruments

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    Immunoaffinity mass spectrometry for leukotriene analysis in brain

    Sources: Cayman Chemical Company, Ann Arbor, Michigan, USA

    Context

    Leukotrienes (LTs) are inflammatory molecules which play an important role in triggering inflammatory diseases such as asthma, neurological diseases and diabetes. As a result, it is important to better understand their role and function in terms of disease progression and their potential value as biomarkers. Due to difficulty of isolation and quantification of these molecules from biological tissues, two different approaches were explored and compared in this study. In both methods, mouse brain tissue homogenates, prepared using the Precellys® Evolution, were used for isolation and quantification of LTs. The results showed that immunoaffinity (IA) enrichment method performed better and proved to be a better sample preparation method for simultaneous measurements of LTs ( LTB(4), LTC(4), LTD(4) and LTE(4)) when compared to the commonly used solid phase extraction (SPE) approach.

    Results

    Table 1. The comparison of Leukotriene concentrations (units) isolated from mice brain homogenates as measured by the indicated method between two extraction methods.

    Table 2. The concentrations (pg/mg) of Leukotriene levels observed in different mouse brain samples. BQL = Below Quantitation Limit.

     

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