Improving compound extraction efficiency using the Precellys 24 - Bertin Instruments

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    Improving compound extraction efficiency using the Precellys 24

    Sources: Stiefel, a GSK Company, North Carolina, USA

    Context

    Determining the skin penetration profile of active pharmaceutical ingredients present in creams, gels, lotions, and ointments is an important aspect of preclinical dermatological research and key to optimizing the selection of formulation prototypes. The aim of this study is to evaluate the use of a high throughput, automated method versus a traditional liquid extraction (LE) technique to extract compounds of interest from skin tissues in a more efficient manner for downstream LC-MS/MS analysis [1]

    Results

    The dermis and epidermis values were compared between the three different extraction methods. The five minute Precellys homogenization procedure showed increased extraction of compound SB-275833 from the dermis samples for all three formulations compared to LE (Figure 1A). For epidermis samples, similarly higher extraction was observed for formulations B and C (Figure 1B)

    Figure 1. Dermal (A) and epidermal (B) amount (μg) of SB-275833 after a 15 and 48 hour liquid extraction (LE) versus homogenization using the Precellys

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