In pre-clinical pharmacology studies, tissue biomarkers are routinely analyzed in our lab. Tissue homogenization is a very critical step to minimize experimental variability in biomarker analysis. To account for biological and experimental variability, biomarker values are generally expressed per mg protein present in homogenates. Cardiac tissue is difficult to homogenize in comparison to brain, spinal cord, liver, kidney etc., and is prone to highly variable protein recovery. We use protein concentration (mg/g tissue) as an indicator of homogenization efficiency. Here we compare cardiac tissue homogenization efficiency using Polytron® and Precellys® homogenizers.
Protein recovery (mg/g tissue) was higher in the cardiac tissue homogenates prepared by the Precellys® homogenizer. A two fold increase in protein concentration was observed in comparison to the Polytron® (Figure 1).
Figure 1. Cardiac muscle homogenization efficiency was compared between the Polytron® and Precellys®. The blue bars represent average protein concentration ± standard error. 30 samples were homogenized on the Polytron® versus 60 samples on the Precellys®, respectively.