Maintaining bacterial viability during tissue homogenization - Bertin Instruments

Microbiology

Maintaining bacterial viability during tissue homogenization

Sources: Illawarra Health and Medical Research Institute, University ofWollongong, Australia

Context

In an animal model of invasive infections caused by Streptococcus pyogenes, bacteria will colonize various tissues and organs. Bacterial loads present in these tissues is determined by homogenization, serial dilution and plating onto nutrient agar. This study investigates the use of the Precellys®24for tissue homogenization.

Results

Following tissue homogenization, the number of viable bacterial cells (CFU/ml) present was determined by serial dilution in sterile 0.7% saline and plating onto nutrient agar. Results (see Figure1) indicate that for all treatment groups the type of bead and the processing time had no significant effect on the viability of S. pyogenes (p =0.1).

 

The effect of tissue homogenization on the viability of S. pyogenes. Data represents the mean CFU/ml from triplicate samples with error bars indicating the standard derivation. The inoculum represents the CFU/ml added to each sample.

The effect of tissue homogenization on the viability of S. pyogenes. Data represents the mean CFU/ml from triplicate samples with error bars indicating the standard derivation. The inoculum represents the CFU/ml added to each sample.

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