Maintaining yeast viability during tissue homogenization with Minilys - Bertin Instruments

Microbiology

Maintaining yeast viability during tissue homogenization with Minilys

Sources: Lab. Microbiologie Fondamentale et Pathogénicité CNRS UMR-5234 - Unité Pathogénicité, Bordeaux, France

Context

Fungi of medical interest in recent years have become primarily responsible for opportunist parasitic diseases, mainly because of the strong increase of immunodepressed patients. Fungal infections pose a challenge to health professionals who have to face variety of new emerging pathogens, but also manage the problem of resistance to antifungal treatments. The team focus its research on yeasts of medical interest belonging to the genus Candida.

Results

Assessment of yeast concentration was performed on the homogenate samples (Results not shown). Effect of homogenization on the viability of yeast cells was performed on a pure Candida culture at 1.107 CFU. Following Minilys homogenization, either performed in 7 mL or 2mL vial, 100 % of yeast cells survived after the maximum of time set independently the tissue. It’s important to limit the temperature rise during long homogenization (>40sec.) by keeping the samples in ice during a few minutes between 40s cycles (Fig.1).

img-03712-810-DU072

Figure 1: Effect of tissue homogenization on the viability of Candida. Data represents the % of yeast survived (CFU/ml) after the duration of homogenization specified.

 

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