Metabolomic and transcriptomic study from fish liver - Bertin Instruments

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Metabolomic and transcriptomic study from fish liver

Sources: Centre for Environment, Fisheries and Aquaculture Science,Cefas Weymouth Laboratory / School of Biosciences, TheUniversity of Birmingham, UK

Context

The goal of this study was to investigate both transcriptomic and metabolomic changes in response to xenoestrogen exposure. Male stickleback fish were used as a model organism for this study as they are environmentally relevant and the stickleback genome draft sequence was available. Key to this study was the ability to carry out both omics techniques upon the same liver tissue homogenate [1] [2].

 

[1] I. Katsiadaki et al., Aquatic Toxicologydoi:10.1016/j.aquatox.2009.07.005[2] H. Wu et al., Anal. Biochem. 37 (2008) 204–212

Results

1H-NMR metabolomics gave highly reproducible data and multivariate analyses suggested a concentration response relationship. Transcriptomic analysis showed induction of hepatic vitellogenins and choriogenins, together with a range of other xenoestrogen-responsive genes. Vitellogenin C was identified as a major responsive isoform, in contrast to other fish species.

 

mRNA expression; one of 48 subarrays of the stickleback 15K cDNA array

mRNA expression; one of 48 subarrays of the stickleback 15K cDNA array

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