The goal of this study was to establish the best protocol of extraction of the whole metabolome of algae. Seaweeds contain a lot of polysaccharides. For instance, in red algae, carrageenans confer a gelling nature to tissue, resulting in difficulties to obtain a reproducible manual grinding. Thus, particular attention has to be paid to grinding and automated techniques to carry out such extraction steps, as cryo-grinding, should be preferred. To avoid metabolite degradation, freeze-drying the sample before grinding is important. Thus, we tested automated cryo-grinding for the metabolite extraction of a red algae, Chondrus crispus, submitted to elicitation by methyl jasmonate (MeJA).
Our results clearly demonstrate the elicitation of the red algae Chondrus crispus by MeJA (results not shown). Morever, the 9 different replicates display very reproducible metabolomic profiles (Figure 1), thanks to simultaneous grinding with Precellys®24. During this study, the Cryolys guaranteed a constant temperature of -20°C within the homogenization chamber and thus avoided an uncontrolled defrosting of the frozen samples.