Metabolomic study of the red algae Chondruscrispus - Bertin Instruments

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    Metabolomic study of the red algae Chondruscrispus

    Sources: MetaboMER, Metabolomic Platform, FR2424 CNRSUPMC,Station Biologique de Roscoff, Roscoff, France

    Context

    The goal of this study was to establish the best protocol of extraction of the whole metabolome of algae. Seaweeds contain a lot of polysaccharides. For instance, in red algae, carrageenans confer a gelling nature to tissue, resulting in difficulties to obtain a reproducible manual grinding. Thus, particular attention has to be paid to grinding and automated techniques to carry out such extraction steps, as cryo-grinding, should be preferred. To avoid metabolite degradation, freeze-drying the sample before grinding is important. Thus, we tested automated cryo-grinding for the metabolite extraction of a red algae, Chondrus crispus, submitted to elicitation by methyl jasmonate (MeJA).

    Results

    Our results clearly demonstrate the elicitation of the red algae Chondrus crispus by MeJA (results not shown). Morever, the 9 different replicates display very reproducible metabolomic profiles (Figure 1), thanks to simultaneous grinding with Precellys®24. During this study, the Cryolys guaranteed a constant temperature of -20°C within the homogenization chamber and thus avoided an uncontrolled defrosting of the frozen samples.

     

    Figure 1: Hierarchical clustering of all detected ions from of control and MeJA elicited Chondrus crispus samples. Samples are in columns; each line represents a detected ion and its variations, from low amount (green) to higher amount (red). Here, only the 91 first ions are represented (2448 detected).

    Figure 1: Hierarchical clustering of all detected ions from of control and MeJA elicited Chondrus crispus samples. Samples are in columns; each line represents a detected ion and its variations, from low amount (green) to higher amount (red). Here, only the 91 first ions are represented (2448 detected).

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