MicroRNA expression profiling of individual rat hypothalamic nuclei - Bertin Instruments

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    MicroRNA expression profiling of individual rat hypothalamic nuclei

    Sources: Neuroendocrinologie Moléculaire de la Prise Alimentaire, University of Paris-Sud 11, UMR 8195, Orsay, France and Neuroendocrinologie Moléculaire de la PriseAlimentaire, CNRS, Centre de Neurosciences, Paris-Sud UMR8195, Orsay, France.

    Context

    MicroRNAs (miRNAs) finely tune messenger RNA (mRNA) expression. As the brain is a highly heterogeneous tissue, physiologically relevant miRNA expression profiling greatly benefits from sampling brain regions or nuclei. MiRNA expression profiling from individual samples is also important for investigating potential differences between animals according to their physiological and pathophysiological status[1].

     

    [1] L.Amar et al., MicroRNA expressionprofiling of hypothalamic arcuate andparaventricular nuclei from single rats using Illumina sequencing technology;Journal of Neuroscience Methods;http://dx.doi.org/10.1016/j.jneumeth.2012.05.033

    Results

    cDNA library sequencing using a GAIIX machine provided individual miRNA expression profiles from single hypothalamic nuclei with a read depth >10-5. Individual miRNA expression profiles are shown for five paraventricular nuclei (PVN). All harbored products from more than 200 miRNA genes, the twenty most abundant of which accounted for 78-85% of the whole profiles (figure 1). Overall, our results showed that cDNA libraries can be constructed with RNAs of 16-30 bases that have been purified from individual rat hypothalamic nuclei.

     

    Figure 1: MiRNA expression in PVN profiles. Expression of the 20 most highly expressed miRNAs are shown for the five PVN profiles. Different miRNAs are shown in different colors.

    Figure 1: MiRNA expression in PVN profiles. Expression of the 20 most highly expressed miRNAs are shown for the five PVN profiles. Different miRNAs are shown in different colors.

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