Mouse UCP-I Quantification in Brown Adipose Tissue - Bertin Instruments

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    Mouse UCP-I Quantification in Brown Adipose Tissue

    Sources: in University of Fribourg, Inst. of biochemistry

    Context

    Our research group is working on circadian rythms and biological clock, especially on their regulations at the molecular level. In the field, Louse Uncoupling Protein 1 (UCP-1) is a mito transmembrane channel protein involved in thermogenesis and only found in brown adipose tissue. This study compares UCP-1 quantity between control mice (that at room temperature during experiment) and mice that are exposed to cold (4°C) during a couple of hours.

    Results

    The lanes correspond to 8 different concentrations of total proteins in order to control the resolution by using the infrared imaging system. UCP-1 quantification studies enable us to get more information about body temperature regulation. Results between control and cold-exposed mice remain confidential.

     

    Detection of lanes with Odyssey Infrared System by using IR-labeled 2nd antibodies. The lanes correspond to 10-30-50-70-20-40-60-80mg concentrations of total proteins.

    Detection of lanes with Odyssey Infrared System by using IR-labeled 2nd antibodies. The lanes correspond to 10-30-50-70-20-40-60-80mg concentrations of total proteins.

     

     

    30 mg of Brown Adipose Tissue from a 3-month old wild type female

    30 mg of Brown Adipose Tissue from a 3-month old wild type female

     

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