Context
This laboratory develops bio-molecular solutions for plant biomass production. Extraction of mycoviral double-stranded RNA (dsRNA) from plant leaves for the investigation of attenuation of Alternaria and Magnaporthe (Ascomycete fungi) infection is a part of the laboratory’s daily work.
Results
A protocol of 5000 rpm x 60 sec with CK28 gave the best results for both green pepper and Malabar spinach leaves homogenization. Extension of the time homogenization attenuated the dsRNA band of mycoviral especially in the Malabar spinach sample.
Agarose gel electrophoresis of 5 mg extracted plant.
