Obtaining a viable single cell suspension with Minilys® prior flow cytometry - Bertin Instruments

    Environment

    Obtaining a viable single cell suspension with Minilys® prior flow cytometry

    Bertin Instruments, Montigny-Le-Bretonneux, FRANCE

    Context

    Flow cytometry is often used to study cells for immunity or cancer research. Obtaining viable single cell suspension is the first step before performing flow cytometry. Although, there are many protocols and methods reporting tissue dissociation, most common techniques are enzymatic and mechanical disruption. The main advantage of mechanical disruption is the ability to contain metabolically active cells, in addition to being faster in comparison with enzymatic approach.
    In this study, a high viability of living cells in a single cell suspension were obtained using bead beating technology operated by the Minilys® within 5 seconds.

    Results



    A. Cell suspension after dying with AOPI (enables the distinction between metabolically active and dead cells)
    B. Viable cells: the green fluorescent calcein is retained by cells with intact membranes
    C. Dead cells: Propidium iodide enters cells with compromised membranes

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