Protein extraction from Arabidopsis with Minilys - Bertin Instruments

Environment

Protein extraction from Arabidopsis with Minilys

Sources: Institute of Plant Molecular Biology, CNRS, Univ. of Strasbourg,France

Context

This institute is presently the largest CNRS centre devoted to integrative plant biology. IBMP focus on the molecular and cellular mechanisms of plant growth, differentiation, development and defense reactions against pathogens and environmental stresses. The research programs use functional genomics, genetics, molecular and cell biology and molecular enzymology.

Results

The total yield of proteins extracted is increasing with buffer extraction and an higher duration of homogenization (Figure 1). Total proteins were separated on a 8% SDSAcrylamide gel and transfer to a PVDF-membrane.

 

Western blot of Arabidopsis total proteins and detection of HD-Zip transcription factor by anti GFPHRP antibody.

Western blot of Arabidopsis total proteins and detection of HD-Zip transcription factor by anti GFPHRP antibody.

 

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