Protein extraction from Mouse Heart Tissue using the Minilys - Bertin Instruments

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Protein extraction from Mouse Heart Tissue using the Minilys

Sources: Antibody Core, Cayman Chemical Company

Context

Cayman Chemical develops and produces antibodies intended for the research market. To ensure quality, suitable tissue lysates are needed to guarantee that our product meets or exceeds our quality control guidelines. As many of our targeted proteins are multi-pass transmembrane receptors, a more robust lysis system is often required.

Results

heart mouse 1

Figure 1: Western blotting using a S1P3 polyclonal antibody (Sphingosine-1-phosphate Receptor 3). The additional band may represent post-translational modification. Lane 1: 3μL Magic Mark Protein Standard. Lane 2: 2.5 μg of S1P3 transfected HEK293 lysate. Lane 3: 5μg of S1P3 transfected HEK 293 lysate. Lane 4: 50μg mouse heart lysate membrane fraction.

 

heart mouse 2

Figure 2: Western blotting using an Optineurin (C-Terminal) polyclonal antibody; Lane 1: 3μL Magic Mark Protein Standard. Lane 2: 60μg of HEK 293 cell lysate. Lane 3: 40μg of HEK 293 cell lysate. Lane 4: 50 μg of mouse heart lysate cytosol fraction. Lane 5 Mouse heart lysate membrane fraction.

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