Protein extraction from mouse soft tissues - Bertin Instruments

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Protein extraction from mouse soft tissues

Sources: Immunotherapix, Paris, France

Context

Immunotherapix is a start up company dedicated towards the development of innovative biologicals for the treatment of anti-inflammatory diseases. To elucidate the anti-inflammatory mechanisms in the preclinical we use mouse models of inflammatory diseases. We analyze tissue and organs samples of injected mice with and without treatment via western blot for the presence or absence of biomarkers for different types of inflammations and immunoregulation. Currently we are extracting proteins either via the Precellys or Minilysbead beater or directly in the tube with a motorised pestle [1].

 

[1] C.Kluge PhD, project leader,c.kluge@immunotherapix.com

Results

The protein recovery was estimated as mg protein in solution standardized to the total weight of the lysed tissue. As reported in the table 1, we obtained excellent results from different mice tissues using the Minilys apparatus for tissue lysis. We also obtained goods results using the Precellys apparatus for tissue lysis. In any case, Precellys based extraction was much more efficient than any other previously used method in our laboratory.

Protein recovery in 100µl buffer volume compared to the initial weight of the lysed spleen and colon tissue obtained with Minilys.

Protein recovery in 100µl buffer volume compared to the initial weight of the lysed spleen and colon tissue obtained with Minilys.

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