Protein extraction from yeast Pichia pastoris with Minilys - Bertin Instruments

Microbiology

Protein extraction from yeast Pichia pastoris with Minilys

Sources: Tokyo University of Agricultural and Technology, Institute ofAgriculture, Division of Bio-regulation and Bio-interaction, Japan

Context

This laboratory develops biomolecular solutions for plant biomass production. The proteins extraction from methylotrophic yeast is a part of the laboratory’s daily work.

Results

The following treatment 4000 rpm 20 sec x 2 already gave good extraction of protein. There were no sign of the excess disintegration of the proteins even after 5000 rpm 20 sec x 6 treatment. Even the 5000 rpm 20 sec x 6 treatment gave no excess heat and the tube was less than 30°C after each treatment.

 

SDS-PAGE of the protein extracted from Pichia pastoris.

SDS-PAGE of the protein extracted from Pichia pastoris.

 

 

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