Caribbean fruit flies (Anastrepha suspensa) are considered a pest of commercial fruit. Ionizing radiation is used as a phytosanitary treatment to disinfect fresh commodities of pests, including Caribbean fruit flies. This treatment renders the insects sterile but may leave some alive. Identifying metabolite biomarkers, such as amino acids, for sterility and eventual death could prove helpful in ensuring the safety of commercial produce.
Pulverization and homogenization of Caribbean fruit flies with the Precellys® Evolution resulted in a uniform extract that cannot be achieved with manual homogenization.
Amino acids were extracted from homogenates with methanol. The methanol supernatant was evaporated and samples were derivatized with a Waters AAA MassTrak kit. Derivatized amino acids were then quantitated by liquid chromatography-mass spectrometry.