Recover live herpes virus from infected mouseganglia with Minilys - Bertin Instruments

    Microbiology

    Recover live herpes virus from infected mouseganglia with Minilys

    Sources: kwang@niaid.nih.gov, MVS, LID, National Institute of Allergyand Infectious Diseases, Bethesda, Maryland, USA

    Context

    The research focus of our laboratory is on human herpes virus pathogenesis and the development of new treatments and vaccines. We often need to test herpes simplex viruses (HSV) and their mutants in a mouse model to study the function of viral genes, evaluate antiviral therapies, and assess the efficacy of vaccine candidates. The titer of infectious HSV in the sensory nerve ganglion innervating the inoculation site is a crucial measurement for these studies.

    Results

    The mouse TG tissue required more than 3 k rpm x 45 sec or 4 k rpm x 20 sec to be adequately homogenized (results not shown). Diluted virus stock (cell free virus) can withstand the homogenization up to 5 k rpm x 60 sec (Fig. 1). Live virus can be recovered from HSV-2 infected mouse TG (Fig. 2).

     

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