RNA extraction from Arabidopsis with Minilys - Bertin Instruments

Environment

RNA extraction from Arabidopsis with Minilys

Sources: Institute of Plant Molecular Biology - CNRS, Univ. of Strasbourg,France

Context

This institute is presently the largest CNRS centre devoted to integrative plant biology. IBMP focus on the molecular and cellular mechanisms of plant growth, differentiation, development and defense reactions against pathogens and environmental stresses. The research programs use functional genomics, genetics, molecular and cell biology and molecular enzymology.

Results

Concentration of RNA obtained from samples after homogenization with Minilys and absorption ratio were determined and shown in the graph 1. An increasing time homogenization and snap-frozen allow higher RNA yield. High RNA quality is obtained from all extracted samples.

 

Graph 1: mean concentration of total RNA from 4 extracted pool samples snap frozen before TRI Reagent addition or not and homogenization with Minilys at 5000 rpm 20s or 40s. The corresponding mean absorption ratio (260/280) are also plotted.

Graph 1: mean concentration of total RNA from 4 extracted pool samples snap frozen before TRI Reagent addition or not and homogenization with Minilys at 5000 rpm 20s or 40s. The corresponding mean absorption ratio (260/280) are also plotted.

 

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