Within the context of The Institute of Cancer Research, ex vivo tumor tissues are being analyzed to study the gene expression of breast tumors and their metastases. Breast cancer xenografts from different cell lines (MDA-MB-435 and GI-101), as well as their lymph node matastases, were frozen in liquid nitrogen after collection.
In collaboration with The Institute of Cancer Research, McElain Laboratories, Sutton UK.
RNA extraction are perfomed following 10 differents protocols :
RNA gel procedure :
0.5 µg of total RNA diluted in 15µl of denaturing loading buffer (containing urea) denatured and run on a 1% agarose gel in 1x TBE at 140v for 1hour.
Gel electrophoresis analysis after the 10 protocols. Efficiency is validated on the primary tumors and lymph node metastases.