RNA extraction from Breast Cancer Xenografts and Lymph Node Metastases - Bertin Instruments

Cancer research

RNA extraction from Breast Cancer Xenografts and Lymph Node Metastases

Sources: at The Institute Cancer Research

Context

Within the context of The Institute of Cancer Research, ex vivo tumor tissues are being analyzed to study the gene expression of breast tumors and their metastases. Breast cancer xenografts from different cell lines (MDA-MB-435 and GI-101), as well as their lymph node matastases, were frozen in liquid nitrogen after collection.

Results

In collaboration with The Institute of Cancer Research, McElain Laboratories, Sutton UK.

RNA extraction are perfomed following 10 differents protocols :

Gel electrophoresis analysis after the 10 protocols. Efficiency is validated on the primary tumors and lymph node metastases.

RNA gel procedure :

0.5 µg of total RNA diluted in 15µl of denaturing loading  buffer  (containing  urea)  denatured  and run on a 1% agarose gel in 1x TBE at 140v for 1hour.

Image27

Gel electrophoresis analysis after the 10  protocols.  Efficiency  is  validated on  the  primary  tumors  and  lymph node metastases.

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